Primer Design for qPCR assays

This course teaches how to pick well designed primers and probes for PCR reactions Introduction: Primers are short oligonucleotide sequences that are designed to hybridize to their complementary targets on the template DNA, to create a PCR amplicon. This course teaches biologists how to design probes and primers for various DNA and RNA based assays or experiments. Why learn Primer Design? Those involved in conducting qualitative or quantitative PCR reactions will know the importance of good initial design principles to ensure success with PCR and to identify reasons for failure or low sensitivity and specificity. Logistics: The course is divided into 4 sessions with pre-recorded videos, handouts, reference cards, examples, data, scripts and quizzes. Enrollees can contact the instructor with questions and get help on the projects. The main topics are listed below, but we teach mostly everything there is to know about NGS analysis. Homework assignments will involve running commands learned in the live lectures. A background in molecular biology is required to understand principles of PCR. Price: $1200 for Commercial/Government enrollees and $900 for Academic researchers and students. Discounts are available for multiple attendees from the same organization or for individuals taking multiple courses. To sign up, click here. Instructor: Syllabus: - Role of short oligonucleotides in biology - Applications-various experimental techniques like PCR, aCGH, cloning, exome-capture - Primer design criteria and characteristics of primers for singleplex and multiplex assays - Primer picking for various types of assays - Target identification and target region selection - ePCR and eDigest